Introduction：

DigestAll NUCLEASE is a genetically engineered endonuclease from Serratia marcescens which is produced and purified from E. coli. The protein is a dimer of identical 245 amino acid, ~30 kDa subunits with two essential disulfide bonds. This promiscuous endonuclease attacks and degrades all forms of DNA and RNA (single stranded, double stranded, linear and circular) and is effective over a wide range of operating conditions. The enzyme completely digests nucleic acids to 5'‐monophosphate terminated oligonucleotides 2–5 bases in length. Although the nuclease is capable of cleavage at nearly all positions along a nucleic acid chain, sequence‐dependent preferences have been demonstrated. The enzyme prefers GC‐rich regions in dsDNA while avoiding d(A)/d(T)‐tracts.

DigestAll endonuclease possess exceptionally high specific activities and is supplied free from measurable protease activities and viral contaminants. It is available at 50 U/ml or at a high concentration (HC) of 250 U/ml. DigestAll is ideal for a wide variety of applications where complete digestion of nucleic acids is desirable.

Unit definition

One unit of DigestAll Nuclease is defined as the amount of enzyme that causes a A260 of 1.0 in 30 min, which corresponds to complete digestion of 37mg of DNA. Note that 1 KU = 1000 units.

Storage:

DigestAll Nuclease is supplied in 50% glycerol containing 50 mM Tris‐HCl pH 8.0, 20 mM NaCl and 2 mM MgCl2. The enzyme preparation is stable for 2 years when stored at –20°C. DO NOT store at –70°C because freezing DigestAll Nuclease results in loss of activity.